| 貨號 | 規(guī)格（ml） | 目錄價（元） |
| T11002 | 0.5 | 1430 |
| T11003 | 1.0 | 2510 |
| T11005 | 3.0 | 6020 |
|
T12006 |
5.0 | 8780 |
Starvio siRNA轉染試劑是我公司研發(fā)團隊在美國西雅圖實驗室研發(fā)成功的一種新型的小核酸轉染試劑。Starvio siRNA可用來轉染siRNA、miRNA mimics、miRNA inhibitor 等200bp以內的小分子RNA，轉染細胞包括絕大多數(shù)貼壁生長的細胞，如一般細胞株、腫瘤細胞株等。目前，普通轉染試劑的主要成分為脂質體或聚乙烯亞胺(Polyethylenimine, PEI)，這兩種成分都具有很大的細胞毒性，并且轉染效果很不理想。Starvio siRNA采用新型可降解納米材料，毒性很低并且擁有非常高的轉染性能，基因敲降效率可高達90%以上，轉染細胞死亡率只有5%左右。血清對Starvio的轉染效果沒有影響，不必使用特殊的低血清培養(yǎng)基，也不必在轉染4-6小時后更換培養(yǎng)液。本產品適用于貼壁細胞株轉染，原代細胞轉染，請選擇StarvioPMsiRNA原代細胞轉染試劑。
產品特點
· 優(yōu)越的細胞轉染性能：絕大多數(shù)貼壁細胞基因敲除效率在90%以上；· 極低的細胞毒性：轉染細胞死亡率在5%左右，大大降低了因細胞毒性對實驗結果的影響；
· 轉染細胞范圍廣，絕大多數(shù)貼壁細胞株都能獲得比較理想的轉染結果。
Fig. 1. Starvio siRNA transfection reagent offers the highest transfection efficiency. HeLa cells were transfected with Alexa 546-dye labeled siRNA at final concentration of 10nM using Starvio sRNA transfection reagent. After 24 hrs, the cells were stained with Hoechst 33342 (nuclear staining dye) and then were viewed by fluorescence microscopy. A The fluorescence images show the fluorescence of labeled siRNA.B.The fluorescence images show the fluorescence of labeled siRNA (in red) overlaid with fluorescence from Hoechst 33342 nuclear staining (in blue).
Fig..2. Starvio siRNA transfection reagent offers the highest level of gene knockdown on a variety of cell types. Different cell lines were transfected with scrambled or Lamin A/C siRNA (final concentration: 10nM) using Starvio In this and all the following figures, all the cells are harvested 48 hours after transfection and Lamin A/C knockdown was measured by qRT-PCR.
Fig..3.Starvio siRNA transfection reagent offers high level of gene knockdown with low toxicity. A549 cell lines were transfected with Lamin A/C siRNA (final concentration: 10nM) using Starvio siRNA transfection reagent. Starvio concentrations added to the cells exceeding transfection concentration of 20 times, the cells still do not produce significant toxicity, which makes these product suitable to optimization. Lamin A/C knockdown was measured by qRT-PCR.
Fig.4.Starvio siRNA transfection reagent offers consistent high level of gene knockdown despite differences in cell density.A549 cells at different cell density were transfected with Lamin siRNA (final concentration: 10nM) using Starvio.
適用細胞
293T（人腎上皮細胞），A549（人肺癌細胞），HEK 293（人胚腎細胞），HeLa（人宮頸癌細胞），MCF-7（人乳腺癌細胞），HepG2（人肝腫瘤細胞），HT-1080(人纖維肉瘤），HLF-a（人肺細胞），Huh-7（人肝癌細胞），KB（人口腔表皮樣癌細胞），PC3（人前列腺癌細胞），BRL-3A（大鼠正常肝細胞），DU 145（人前列腺癌細胞），Hepa 1-6（小鼠肝癌細胞），OVCAR3（人卵巢癌細胞），NCaP-FGC（人前列腺癌細胞），C2C12（成肌細胞），MDCK（犬腎細胞），CHO-K1，islk.219細胞（人肺癌細胞），HT-29（人結腸癌細胞），NCI-H1688（人典型小細胞肺腫瘤/癌細胞），RKO結腸癌細胞，Vero（非洲綠猴腎細胞），COS-1（非洲綠猴腎成纖維細胞），COS7-L（非洲綠猴腎成纖維細胞），COS-7（非洲綠猴腎成纖維細胞），SK-BR3（人乳腺癌細胞），Hepa 1cLc7（鼠肝癌細胞），Daoy（人髓母細胞瘤細胞），DBTRG-05MG（人腦膠質母細胞瘤細胞），KLN 205（鼠肺磷癌細胞），Neuro-2a（小鼠神經瘤母細胞），DI-TNC1（大鼠腦間質細胞）等。
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